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HPLCBASIC
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
What is
HPLC?
Chromatographic Process.
HPLC Equipment
Resolution: The two equations, the three parameters.
Column Efficiency.
USP tailing.
Peak Dispersion:
Mass Transfer, Eddy Diffusion, Longitudinal Diffusion.
Introduction
to ChemStation.
HPLC
stands for HIGH PERFORM LIQUID CHROMATOGRAPHY.
HPLC
definition can be divide in three parts with purpose to be more clear the
explanation.
1st
- It is a separation technique, which separates the
individual components.
2nd
- Identify the individual components - Qualitative
Analysis.
3rd
- Assay the components - Quantitative Analysis.
CHROMATOGRAPHIC
PROCESS:
THIN LAYER
CROMATOGRAPHY (TLC)
REVERSE PHASE
CHROMATOGRAPHY (RPC)
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